marimammalium, we propose that group M strains should be classifi

marimammalium, we propose that group M strains should be classified as a new species (Stackebrandt et al., 2002). DNA relatedness among the group M strains was>73.1%. Thus, these three strains were confirmed to be the same species. Group M strain PAGU1330 from a human subject was located within the Mitis group with Streptococcus infantis being the closest species in the phylogenetic analysis (16S rRNA gene sequence similarity, 98.7%). The group M strains of canine origin were Gram-positive cocci and occurred in pairs or short chains. These organisms were facultatively

Enzalutamide mw anaerobic and catalase negative. The colonies that they formed were generally small and translucent on blood agar. In the biochemical test, these strains with group M antigens closely resembled each other. β-Galactosidase activity and utilization of glycogen could distinguish them from the closely related species (Table 2). The G+C content of the DNA of PAGU 653 was determined to be 38.4±0.3 (mean±SD) mol%, which is within the characteristic range of the genus Streptococcus Androgen Receptor activity (34–46 mol%) (Spellerberg & Brandt, 2007). This value is similar to those of other close phylogenetic relatives (e.g. S. marimammalium, 38.0 mol%; S. phocae, 38.6 mol%; Streptococcus castreus, 37.4 mol%) (Skaar et al., 1994; Lawson et al.,

2005a, b). The group M streptococci was established by Fry in 1941 (personal communication cited from Wilson & Miles, 1955). Only the β-hemolytic group M strains isolated from the animal Nintedanib (BIBF 1120) (the tonsil of the dog) were recognized until 1955 (Wilson & Miles, 1955). However in 1959, Skadhauge & Perch (1959) reported the α-hemolytic human strains of group M isolated from the gingival mucosa of healthy persons or from the blood of patients suffering from subacute bacterial endocarditis. They proposed the three biovars within the group M streptococci; biovar-I consists of α-hemolytic human strains that

fail to hydrolyze arginine and have a final pH in glucose broth of 4.6–5.2. Biovar-II strains are of animal origin, β-hemolytic, hydrolyze arginine and attain a final pH of 6.3–7.2. Biovar-III strains are also of animal origin, β-hemolytic, hydrolyze arginine but produce more acid from glucose (final pH 5.9–6.7). Broome et al. (1976) also report many group M α-hemolytic human strains, isolated from the patients of endocarditis, or septicemia from a sternal abscess. In this study, we used only one human isolate called ‘Lindstrøm’ (=PAGU 1330), which was stated as a group M biovar-I strain (Skadhauge & Perch, 1959). The phylogenetic position of the strain was located within the Mitis group and not with the canine, β-hemolytic strains (Fig. 1). Colman (1968) stated that some strains of group M resembled ‘Streptococcus viridans’ or Streptococcus mitis, which would indicate the biovar-I strain group, namely α-hemolytic human group M strains. Additional experiments to determine the accurate phylogenetic and taxonomic position of the biovar-I strain group are required.

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