1-ploid mutant or wild-type viral genome. Results: HBV rtA181S mutation was detected in 98 nucleos(t)ide analog(NA)-expe-rienced patients by direct sequence analysis, representing 0.53 %(98/18,419) across the study

population and 0.86 %(46/5,344) in the patients who were receiving ADV at the resistance testing. By contrast, signature ADV-resistant mutations rtA181V and/or rtN236T were detected in 1,311 patients, representing 7.12 %(1,311/18,419) of the study population LBH589 mouse and 24.53 %(1,311/5,344) of the patients who were receiving ADV at the resistance testing. Genotype C and genotype B HBV infection occupied 91.8 %and 8.2 %in rtA181S-positive patients, in contrast to 84.6 %and 15.4 %in rtA181S-negative patients (P <0.01). All rtA181S-positive patients had received NA treatment, including single

lamivudine (LAM) (15.3%), single ADV (20.4%), LAM switching to/add-on ADV (13.3%), LAM switching to entecavir (ETV) (9.2%), LAM switching to ADV and then switching to ETV (11.2%), and other antiviral therapy schedules (30.6%). rtA181S was detected in multiple patients with virologic breakthrough (including 7 patients with single rtA181S). Phenotypic analysis of patient-derived viral strains showed that AZD2281 in vitro rtA181S, rtA181S+N236T, rtN236T and rtA181V strains had 68.5%, 49.9%, 71.4 %and 66.2 %of natural

replication capacity of wild-type strain, and 3.7-, 9.8-, 7.9- and 5.4-fold increased EC50 to ADV. The rtA181S strain remained susceptible to LAM, ETV and tenofovir, and ADV susceptibility was restored after the mutation was eliminated through site-directed mutagenesis. Consistently, rescue therapy with ETV or combination of ETV and Dolichyl-phosphate-mannose-protein mannosyltransferase ADV was effective for rtA181S-related ADV-refractory patients in clinical observation. Conclusion: The rtA181S mutation primarily confers moderate resistance to ADV. It could be induced by either LAM or ADV but only contribute to ADV resistance. Disclosures: Vincent W. Wong – Advisory Committees or Review Panels: Abbvie, Gilead; Consulting: Merck, NovaMedica; Speaking and Teaching: Gilead, Echosens Henry Lik-Yuen Chan – Advisory Committees or Review Panels: Gilead, MSD, Bristol-Myers Squibb, Roche, Novartis Pharmaceutical; Speaking and Teaching: Echosens, Abbvie The following people have nothing to disclose: Yan Liu, Xiaodong Li, ShaoJie Xin, Zhihui Xu, Rongjuan Chen, Jing Yang, Li Chen, Dongliang Yang, Dongping Xu “
“Protein tyrosine phosphatase receptor type O (PTPRO), one of the receptor types of phosphotyrosine phosphatases (PTP), was recently described as a tumor suppressor in various kinds of cancers. We aimed to clarify the role of PTPRO in hepatocellular carcinoma (HCC).