The water was ultrapure water obtained

from a Milli-Q-system (Millipore Corporation, Bedford, MA, USA) and nitric acid (68 – 70%), hydrochloric acid (30%), ammonium Galunisertib molecular weight carbonate (powder), hydrogen peroxide (30%) and formic acid (98%) were all from J. T. Baker (Deventer, Netherlands). In the arsenic speciation analysis arsenobetaine (AB) (Fluka Analytical, Italy), arsenic(III)oxide (As(III)) (Aldrich Chemistry, USA), dimethyl arsenic acid (DMA) (Chem Service, USA), monomethyl arsenic acid disodium salt (MMA) (Argus Chemicals, Italy) and arsenic(V) (As(V)) standard solution (Merck, Germany) were used. Two stock solutions of each standard compound were made; for AB, As(III), DMA and MMA the concentrations were 100 mg/L and 1 mg/L and for As(V) the concentrations were 10 mg/L and 0.1 mg/L. The stock solutions were prepared in nitric acid (1%), with the exception of As(III), in which concentrated hydrochloric acid was used to promote its dissolution. The final standard concentrations for all compounds were 1, 5, 10, 20 and 50 μg/L in 1% nitric acid. Three standard stock solutions for the ICP-MS analysis were prepared 100, 10

and 1 μg/L from ICP Calibration mix FS9 ME175 multielement reference solution (Romil, Cambridge, GB). From these stock solutions, seven standard solutions were made (0.005, 0.01, 0.05, 0.1, 0.5, 1 and 16 μg/L). The stock solutions and final standard solutions were both prepared in selleck chemicals llc 2% nitric acid. In final SB-3CT standard solutions, internal standard, rhodium (Romil, Cambridge, GB), was incorporated. A stock solution of 1 mg/L rhodium was made daily in ultrapure water. The stock solution was added to

final standards and samples so that the final concentration of rhodium was always 10 μg/L. In the total arsenic determination, a quadrupole inductively coupled plasma mass spectrometer (Thermo Fisher Scientific XSeries II, Waltham, Massachusetts, USA) was used. In the inorganic arsenic analysis, the ICP-MS was equipped with a high performance liquid chromatograph (Waters 2690 Separations Module, Waters, USA). An anion exchange column Hamilton PRP-X100 (Bonaduz, Switzerland), 250 × 4.6 mm 5 μm, and pre-column, 25 × 2.3 mm, were used to separate the arsenic species. Sample preparation was performed in a microwave oven (Milestone Ethos Plus High Performance Microwave Lab station, Chelton, Connecticut, USA). Long grain rice samples were homogenised before microwave assisted digestion in the presence of strong nitric acid (3 mL), hydrogen peroxide (2 mL) and ultrapure water (3 mL). The sample was weighed (0.5 g) into a digestion vessel and the reagents were added. The microwave digestion program was as follows: 5 min to 100 °C, 5 min to 130 °C, 5 min to 160 °C, 7 min to 200 °C, 10 min at 200 °C and cooling down to 80 °C.