, 2004) and possibly also due to

old forests becoming denser ( Gauslaa et al., 2007). It is today found in small and isolated populations, and is red-listed in several countries, among them Sweden ( Gärdenfors, 2010). The species is commonly used in lichen transplant experiments (e.g. Scheidegger, 1995, Gaio-Oliveira et al., 2004 and Gauslaa et al., 2006). It has also since almost two decades been used as an indicator species to identify forest habitats with high conservation value in Sweden, as field experience has shown that it reflects the presence of other uncommon and declining species ( Nitare, 2005). There are also indications INK 128 clinical trial that the species may reflect high conservation values at the landscape scale ( Kalwij et al., 2005). At the initiation of our transplantation experiment in 1994, L. pulmonaria was not red-listed in Sweden ( Databanken för hotade arter and Naturvårdsverket, 1990). The study area is located in the hemi-boreal zone (Ahti et al., 1968) in East-Central Sweden (60°02′N, 18°22′E). The proportion of forest GSK-3 inhibitor >80 years old in the region is 24%, with Norway spruce Picea abies (L.) H. Karst. and Scots pine Pinus sylvestris L. being the dominant tree species, but the proportion of aspen is unusually high, 4% ( Swedish Forest Agency, 2012). Altogether 1120 pieces of L. pulmonaria, each about 6 cm2

large, were transplanted in spring and autumn of 1994 to 280 aspens at 35 sites ( Table 1). Each site consisted of a forest and a clearcut, with four receiver aspen in each, i.e. altogether eight trees per site. In 19 clearcuts the receiver trees were solitary (scattered) and in 16 sites they occurred in groups of broad-leaved

trees (grouped: >3 aspens >18 cm diameter at breast height and <15 m from each other). The 35 sites were situated within an area of 1900 km2, with an average distance between them of 24.7 km (range 0.4 - 65 km). In spring as well as in autumn of 1994, two Methocarbamol transplantations were made per tree, one on the north and one on the south side of the stems 140 to 180 cm above ground level, amounting to a total number of four transplants per tree. The thallus pieces were attached to the stem with the help of a plastic net (6 × 6 cm with 1 × 1 cm meshes) and metal staples to the bark. Each sample was sprayed with tap water immediately after transplantation. All transplantation sites were visited in summer 1996 and spring 2008 to visually evaluate survival and vitality of the transplants. Prior to evaluation, transplants were sprayed with water in order to enable relevant comparisons since dry and wet L. pulmonaria thalli differ in color. If any thallus part remained, the transplant was judged as having survived. If ⩾50% of a survived thallus was in a viable condition (i.e. giving a healthy impression with a green, intact surface without necrosis or signs of damage), the transplant was assessed as being vital.