11. Le FP, Jacques I, Grayon M, Al DS, Bouchon P, Denoeud F, Nockler K, Neubauer H, Guilloteau LA, Vergnaud G: Evaluation and selection of selleck kinase inhibitor tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol 2006, 6:9.CrossRef 12. Tiller RV, De BK, Boshra M, Huynh LY, Van Ert MN, Wagner DM, Klena J, Mohsen TS, El-Shafie SS, Keim P, Hoffmaster AR, Wilkins PP, Pimentel G: Comparison of two multiple-locus variable-number tandem-repeat analysis methods for molecular strain typing of human Brucella melitensis isolates from the

Middle East. J Clin Microbiol 2009, 47:2226–2231.PubMedCrossRef 13. Valdezate S, Navarro A, Villalon P, Carrasco G, Saez-Nieto JA: Epidemiological and phylogenetic analysis of ML323 mw Spanish human Brucella melitensis strains by multiple-locus

variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpoB typing. J Clin Microbiol 2010, 48:2734–2740.PubMedCrossRef 14. Kattar MM, Jaafar RF, Araj GF, Le FP, Matar GM, Abi RR, Khalife S, Vergnaud G: Evaluation of a multilocus variable-number tandem-repeat analysis scheme for typing human Brucella isolates in a region of brucellosis endemicity. J Clin Microbiol 2008, 46:3935–3940.PubMedCrossRef 15. Marianelli C, Petrucca A, Pasquali P, Ciuchini F, Papadopoulou S, Cipriani P: Use of MLVA-16 typing to trace the source of a laboratory-acquired Brucella infection. J Hosp Infect 2008, 68:274–276.PubMedCrossRef 16. Garcia-Yoldi D, Le FP, Marin CM, de Miguel MJ, Munoz PM, Vergnaud G, Lopea-Goni I: Assessment of genetic stability of Brucella melitensis Rev 1 vaccine find protocol strain by multiple-locus variable-number tandem repeat analysis. Vaccine 2007, 25:2858–2862.PubMedCrossRef 17. Alton GG, Jones LM, Pietz DE: Laboratory techniques in brucellosis. Monogr Ser World Health Organ 1975, 1–163. Dynein 18. Bricker BJ, Halling SM: Differentiation

of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 1994, 32:2660–2666.PubMed 19. Hunter PR, Gaston MA: Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol 1988, 26:2465–2466.PubMed Authors’ contributions JH did most of the typing work and wrote the report. ZHY, TGZ and PDR prepared the DNA samples. FMG, MJC and YRP were in charge of epidemiological investigation and collection of Inner Mongolia strains. CJD, KCW and DXL were in charge of epidemiological investigation and collection of Guangdong strains. CBY managed the project. All authors read and approved the final manuscript.”
“Background Legionella bacteria are ubiquitous in nature and are often found in natural water sources as well as in man-made water systems. Humans may be infected through inhalation of contaminated aerosolised water droplets. Symptoms range from influenza-like disease (Pontiac fever) to severe pneumonia (Legionnaires’ disease, LD) with a high mortality rate [1, 2].