2007). Notes: Penicillium steckii was described by Zaleski (1927) and accepted by Raper and Thom (1949) and Ramirez (1982), but was placed by Pitt (1979) in synonymy with P. citrinum. Pitt (1979) broadened the concept of P. citrinum for P. steckii and noted that strains of this species do not produce citrinin and are not able to check details grow at 37°C. This study shows that this is sufficient to raise these isolates to species level. Penicillium corylophiloides was described without a Latin diagnosis and designation of a holotype specimen (Abe

1956). After its description, this species was placed in synonymy with P. corylophilum by Smith (1963), while Pitt (1979, 2000) placed this species in synonymy with P. jensenii. Abe (1956) noted that P. corylophiloides formed typically elliptically formed conidia, in contrast with P. citrinum and P. steckii. However, our analysis showed that P. steckii also forms broadly ellipsoidal conidia. Following the phylogenetic species concept, P. steckii and

P. corylophiloides are separated species; however, no differences in morphology, physiology or extrolites patterns could be observed between these species and are therefore placed in synonymy. Further work should show if these are two distinct species. Penicillium tropicoides Houbraken, Frisvad and Samson, sp. nov.—MycoBank MB518293; RXDX-101 Fig. 6. Fig. 6 Penicillium tropicoides. a-c Colonies grown at 25°C for 7 days, a CYA, b MEA, c YES, d-e sclerotia,

f-g ascospores, h-i conidiophores, j conidia.—scale bar = 10 μm, except f. = 1 μm Etymology: The new species is related to P. tropicum. Eupenicillio tropico affine, sed coloniis 30°C tarde et 38°C haud crescentibus, cleistotheciis griseo-brunneis abundantibus, maturescentibus post tres menses; isochromantoxina formantur. Holotype: CBS 122410T is designated here as the holotype of Penicillium tropicoides, isolated DNA ligase from soil of a rainforest, near Hua-Hin, Thailand. Description: Colony diameter, 7 days, in mm: CYA 24–30; CYA30°C 12–18; CYA37°C no growth; MEA 18–23; YES 36–43; CYAS 31–39; creatine agar 13–16, poor to moderate growth and weak acid production (under colony). Cleistothecia abundantly produced on CYA and drab grey coloured; conidia sparsely produced, blue grey green, colonies typical with large hyaline exudate droplets, reverse on CYA crème-brown, soluble A-1210477 ic50 pigments absent. Weak sporulation on YES, cleistothecia abundant present and drab-grey in colour, soluble pigment absent. Colonies on MEA ascomatal, in shades of grey. No reaction with Ehrlich test. Cleistothecia sclerotioid, 200–300 μm in diameter, ripening slowly and mature after 3 months on MEA and Oatmeal agar. Ascospores ellipsoidal, \( 2.4 – 3.2 \times 1.7 – 2.