60 ± 5.33 13.33 ± 7.42 10.79 ± 7.84 (μg·kg-1) CHO 11.00 ± 8.68 9.23 ± 7.60 10.44 ± 8.00 Interleukin 2 and interleukin 5 responses Resting IL-2 was significantly higher in CHO than in P (p = 0.028; Table  3). Therefore, resting IL-2 measures were entered as a covariate in a 2×2 (treatments x time) repeated measures ANCOVA. Using this comparison, IL-2

was unchanged after RE (time effect p = 0.359). There were no differences between CHO or P in IL-5 (treatment x time interaction p = 0.610). IL-5 4EGI-1 cell line was significantly decreased after RE (time effect p = 0.040). Specifically, IL-5 was significantly (−37%) lower than resting levels at 90 min post (p = 0.008). Table 3 Interleukin-2 and interleukin-5 response to resistance exercise with carbohydrate ingestion or placebo (n=7) Variable Condition Pre Post 60min Recovery Interleukin 2 PLC Dinaciclib concentration 4.62 ± 6.42* 6.14 ± 12.32 20.88 ± 29.63 (pg·ml-1) CHO 64.04 ± 54.52* 36.89 ± 18.82 11.63 ± 9.90 Interleukin 5 PLC 1.73 ± 0.61 1.07 ± 0.38 0.60 ± 0.70 (pg·ml-1) CHO 1.67 ± 0.32 1.43 ± 0.30 1.09 ± 0.47 *indicates p<0.01 difference between conditions. Discussion Despite the tremendous growth of investigations regarding the impact of endurance exercise on immune parameters, still less is known about the effects of resistance exercise. Several investigations suggest that reduced levels

of S-IgA are associated with an increased risk of URTI during periods of heavy training, and it has been suggested that CHO supplementation may influence immune indices in response to heavy exertion. The purpose of this investigation was to determine whether carbohydrate ingestion prior to-, during and following

RE would alter the immune response to RE. Ours was the first study to examine s-IgA and cytokine responses using paired-exercises, which buy Ilomastat lasted over 30 min, selleck compound shown to elicit a greater stress and immune response [18]. We hypothesized that CHO ingestion would result in a lesser perturbation in s-IgA and circulating cytokines from resting values as compared to placebo. The major findings of this study were: 1) resistance exercise did not result in measureable changes in s-IgA or IL-2 responses; 2) a significant reduction in IL-5 responses were observed; 3) contrary to our hypothesis, CHO supplementation prior to-, during, and following RE had no effect on immune responses. These findings help to clarify what has been previously unknown in this area. The central premise behind our hypothesis was that carbohydrate ingestion would blunt the rise of epinephrine and norepinephrine during RE, and thus alter s-IgA and circulating cytokines measured as compared to control. Some previous studies [22] of carbohydrate ingestion during exercise have found significant reductions in epinephrine and norepinephrine while others have found no effect [28]. Thus the impact of carbohydrate ingestion on the catecholamine response to exercise appears to be variable.