70 ± 0.04 0.18 ± 0.01 3.53 ± 0.01 0.11 0.05 HpyCH4V TGCA 3.85 ± 0.75 3.70 ± 0.03 3.45 ± 0.03 www.selleckchem.com/products/azd5363.html 3.53 ± 0.03 1.04 0.98 HpyCI GATATC 0.00 ± 0.03 0.31 ± 0.01 0.02 ± 0.00 0.33 ± 0.00 0.01 0.07 HpyF10VI GCNNNNNNNGC 2.70 ± 0.35 1.96 ± 0.04 2.97 ± 0.09 1.43 ± 0.02 1.38 2.07 HpyF14I CGCG 2.26 ± 0.46 1.96 ± 0.05 1.55 ± 0.05 1.43 ± 0.02 1.15 1.08 HpyF2I CTRYG 1.16 ± 0.17 0.92 ± 0.01 0.37 ± 0.01 0.88 ± 0.00 1.26 0.42 HpyF36IV GDGCHC 0.20 ± 0.21 1.22 ± 0.03 0.31 ± 0.01 0.93 ± 0.01 0.16 0.33 Hpy44II GGNNCC 1.21 ± 0.38 1.96 ± 0.05 0.44 ± 0.00

1.43 ± 0.02 0.62 0.31 HpyII GAAGA 2.29 ± 0.23 2.14 ± 0.03 2.87 ± 0.02 2.16 ± 0.00 1.07 1.33 HpyIP CATG 4.63 ± 0.25 3.70 ± 0.03 4.43 ± 0.04 3.53 ± 0.01 1.25 1.25 HpyIV GANTC 1.70 ± 0.25 3.70 ± 0.04 1.66 ± 0.02 3.53 ± 0.01 0.46 0.47 HpyNI CCNGG 2.04 ± 0.30 1.96 ± 0.05 0.87 ± 0.02 1.43 ± 0.02 1.04 0.61 HpyPORF1389P GAATTC 0.01 ± 0.05 0.31 ± 0.01 0.11 ± 0.00 0.33 ± 0.00 0.03 0.32 HpyV TCGA 0.95 ± 0.25 3.70 ± 0.03 0.18 ± 0.00 3.53 ± 0.01

0.26 0.05 HpyVIII CCGG 1.92 ± 0.30 1.96 ± 0.04 1.06 ± 0.02 1.43 ± 0.02 0.98 0.74 aRestriction endonucleases with palindromic recognition sites are indicated in bold. Code of degenerate nucleotide letters: R = G or A; Y = C or T; S = G or C; W = A or T; D = not C (A or G or T); H = not G (A or C or T); N = any nucleotide. bO/E ratio https://www.selleckchem.com/products/gsk2126458.html indicates the observed/expected (O/E) ratio values. cExclusively underrepresented

in hpEurope LY411575 MLS. The observed/expected (O/E) ratio indicates deviation from the expectation based on G + C ratio. O/E ratios were highly similar for the WGS and MLS (R2 = 0.87, p < 0.001), without any differences by haplotype. Analysis of the hpEurope and hspAmerind sequences showed that 10 of the 32 cognate restriction sites were underrepresented in MLS and 6 of those sites were also underrepresented in WGS (defined as O/E ≤ 0.5 and Chi Square p-value ≤ 0.005; Table 2). One exception, Hpy166III (cognate site: CCTC) was exclusively underrepresented in hpEurope MLS, but not in the hspAmerind nor in WGS. The underrepresented sites ifenprodil varied in their C + G content from 33.3 to 75%. Most (9) of those 10 underrepresented sites were palindromic [28–30] (Table 2). Conversely, only one cognate recognition site: Hpy99III (cognate site: GCGC), was strongly overrepresented (O/E ≥ 2 and Chi Square p-value ≤ 0.005) in both hpEurope/hspAmerind MLS and WGS (Table 2).