DCAL carried out some of the molecular genetic studies.

EMF helped with sampling and processing steps. LQF and GRP helped with anaerobic manipulation of samples and design of the experiments. MJM participated in the data interpretation. CH participated in the data interpretation and writing. RSP helped in the experiment design, data interpretation and wrote the manuscript. RMCPD and ASR were the major responsible by the experiment JQ1 cost design, and helped in data interpretation and wrote the manuscript. All authors read and approved the final manuscript.”
“Background Leptospirosis is a common mammalian zoonosis occurring worldwide. The causative agents are different serovars of pathogenic Leptospira strains, bacteria that belong to the order Spirochaetales. They can affect humans as well as a wide range of different mammals [1] while the check details clinical manifestations differ considerably [2, 3]. In dogs [4–6] and humans [7, 8] clinical signs vary from self-limiting flu-like symptoms to a severe illness with manifestation

in specific organs, including the kidneys with acute renal failure [9], which can lead to death. In pigs [10, 11] and cattle [12] still birth, abortion, and foetal birth deformities may occur. In horses Leptospira spp. play a role in the clinical manifestation of the Equine Recurrent Uveitis (ERU) [13]. The systematic classification of Leptospira spp. is complex, since the traditional classification is based on the undefined antigenic diversity between serovars [3]. This system divides the genus Leptospira Linsitinib supplier in two groups: Leptospira interrogans sensu lato including all pathogenic strains and Leptospira biflexa sensu lato representing all non-pathogenic and saprophytic strains. Genetic classification is based on DNA

hybridization and a wide range of DNA sequencing methods. Twenty genomospecies are currently described Dichloromethane dehalogenase [14, 15]. Since immunological and genetic typing methods target different cellular structures, these classification systems do not correspond [15]. Consequently, the characterization of Leptospira spp. is still challenging and time-consuming. The most commonly used diagnostic tool for clinical samples is antibody detection by the microscopic agglutination test (MAT). If serum antibodies against Leptospira spp. are present in a clinical sample, they will agglutinate with viable, cultured organisms of specific Leptospira serovars [16]. This test is highly sensitive and specific provided that the panel of bacteria used represents the specific regional epidemiological status regarding pathogenic strains. Furthermore, it is well-described that different outcomes of MAT results can occur when they are performed in different laboratories and with different MAT panels, underlining the need of internal controls [17, 18]. Several molecular methods have been established to detect leptospiral DNA using specific targets to trace the agents in clinical samples such as urine.