Here, we investigate whether cuticular hydrocarbons, chemical compounds found on the cuticle of most terrestrial arthropods, provide a means of discerning genetic similarity during mate choice in the cricket, Teleogryllus oceanicus. We found IPI-549 mouse that individuals preferentially mated

with partners who share more dissimilar cuticular hydrocarbon profiles and that similarity in cuticular hydrocarbon profiles between mating pairs correlated with their genetic similarity. Our results provide good evidence that cuticular hydrocarbon profiles offer a means of assessing genetic compatibility in T. oceanicus, enabling individuals to choose their most genetically suitable mate.”
“Introduction: Previous studies in animal models of osteoarthritis suggest that alendronate (ALN) has antiresorptive and chondroprotective effects, and can reduce osteophyte formation. However, these studies used non-physiologic injury methods, and did not G418 inhibitor investigate early time points during which bone is rapidly remodeled prior to

cartilage degeneration. The current study utilized a non-invasive model of knee injury in mice to investigate the effect of ALN treatment on subchondral bone changes, articular cartilage degeneration, and osteophyte formation following injury. Methods: Non-invasive knee injury via tibial compression overload or sham injury was performed on a total of 90 mice. Mice were treated with twice weekly subcutaneous injections of low-dose ALN (40 mu g/kg/dose), high-dose ALN (1,000 mu g/kg/dose), or vehicle, starting immediately after injury until sacrifice at 7, 14 or 56 days. Trabecular bone of the femoral epiphysis, subchondral cortical bone, and osteophyte volume were quantified using micro-computed tomography AZD3965 ic50 (mu CT). Whole-joint histology

was performed at all time points to analyze articular cartilage and joint degeneration. Blood was collected at sacrifice, and serum was analyzed for biomarkers of bone formation and resorption. Results: mu CT analysis revealed significant loss of trabecular bone from the femoral epiphysis 7 and 14 days post-injury, which was effectively prevented by high-dose ALN treatment. High-dose ALN treatment was also able to reduce subchondral bone thickening 56 days post-injury, and was able to partially preserve articular cartilage 14 days post-injury. However, ALN treatment was not able to reduce osteophyte formation at 56 days post-injury, nor was it able to prevent articular cartilage and joint degeneration at this time point. Analysis of serum biomarkers revealed an increase in bone resorption at 7 and 14 days post-injury, with no change in bone formation at any time points. Conclusions: High-dose ALN treatment was able to prevent early trabecular bone loss and cartilage degeneration following non-invasive knee injury, but was not able to mitigate long-term joint degeneration.