infantarius

BAA-102 and S. gallolyticus UCN34), and four segment 16S rRNA genes (EU163500, EU163502, EU163503, and EU163504) in the S. bovis group were selected for Selleckchem LCZ696 an evolutionary study. The reference strain of S. lutetiensis (accession number: EU163503) was found to be the nearest strain to the S. lutetiensis genome sequence in our study, showing the same 16S rRNA gene sequences. Compared with the nearest species S. infantarius subsp. infantarius BAA-102 and EU163504, strain 033 had two and three nucleotide differences in the 16S rRNA genes, respectively. An entire genome

comparative analysis was performed on the four completed genomes of S. gallolyticus subsp. gallolyticus BAA-2069, S. gallolyticus subsp. gallolyticus ATCC43143, and S. gallolyticus subsp. pasterurianus ATCC43144 in the S. bovis group. selleck chemical The S. lutetiensis sequenced genome in our study was found to be phylogenetically related to the genome of S. gallolyticus subsp. pasterurianus ATCC43144; and 94.1% of the genes were found in the homologous genes in ATCC43144 (Figure 5A) [14]. Although large-scale genome rearrangements, inversions and deletions were observed, the four genomes displayed the same collinear structure (Figure 5B). We found 15.2% of the genes of S. gallolyticus subsp. pasterurianus and 34.9% of the genes of S. gallolyticus subsp. gallolyticus were not present in S. lutetiensis, suggesting that the Oxalosuccinic acid genome of S. lutetiensis strain 033 was similar to that of S. gallolyticus subsp. pasterurianus (Figure 5A). Discussion Selective media are routinely

used to isolate particular pathogens from mixtures of bacterial species from the feces of patients with diarrhea. GDC-0994 price However, they cannot be used to isolate putative bacterial agents of diarrhea of unknown etiology. The important feature of the direct sequencing of the 16S rRNA gene in the fecal samples is the ability to identify most of the existing bacterial species [33]. Using this technique, we analyzed the dynamics of the fecal bacteria flora in nine patients with diarrhea of unknown etiology. We examined three fecal samples per patient, at admission, during recovery, and after recovery.