P. fluorescens Pf0-1 has specific genetic responses to different soil types, but also general mechanisms required for
persistence. Our observation that sif2 is important in two distinct soil types points to a general phenomenon in which bacterial responsiveness to nitrogen and its shunting into central metabolism via glutamine in situ is critical for fitness. This concept is further supported by the observation that several of soil-activated sequences are associated with putative σ54 promoters. Thus, a general key element in bacterial Epigenetics inhibitor adaptation to soils is to maintain nitrogen homeostasis. Acknowledgements This work was supported in part by the Agriculture and Food Research Initiative Competitive Grant 2010-65110-20392 from the USDA’s National Institute of Food and Agriculture, Microbial Functional
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