The phage-infected fermentation broth had to be discharged after chemical treatment, and no effective means of salvaging phage-contaminated fermentation broths were ever developed. Herein, feeding seed culture to the fermentation broth was learn more proposed as an effective
remedial action and shown in Figure 8. Figure 8 Effect Transmembrane Transporters activator of feeding seed cuture for phage infection in the 2-Keto-Gluconic Acid (2KGA) fermentation process. As for the infection of phage KSL-1 at 0th hour, when cell concentration decreased to 2.07 g/L at the 20 h of fermentation, fresh seed culture was fed. 2KGA fermentation continued to the endpoint with the produced 2KGA concentration of 159.89 g/L, which was 1.11 times of that infected fermentation at 0th hour without seed culture feeding. The total fermentation time decreased to 80 h with the complete consumption of glucose, and the productivity and yield of 2KGA increased to 2.0 g/L.h and 0.89 g/g. Interestingly, cell concentration showed a waving model which may contribute to the bacterial succession and co-evolution of bacteria and their viruses in an arms race [22]. When feeding fresh seed culture into the 8th -h infected fermentation broth, fermentation time decreased
to 72 h which comparable to the normal process. 2KGA concentration increased slightly from 168.85 g/L to 171.34 g/L. Table 1 summarized the overall fermentation performances of 2KGA production under the conditions of normal and phage infection with/without feeding fresh seed culture at various infection stages. Therefore, feeding fresh seed culture to infected fermentation broth was proposed once the cell Eltanexor mw concentration began to decrease after phage infection. And this proposed remedial action was effective to obtain the desirable 2KGA fermentation performance without stopping the 2KGA production process and discharging the infected broth. Table 1 Summary of 2KGA production from phage infection at different stages by Pseudomonas fluorescens K1005 Parameters Without feeding seed cuture With feeding seed cuture Normal Infected phage at 0 h Infected phage at
4 h Infected phage at 8 h Infected phage Phospholipase D1 at 0 h Infected phage at 4 h Infected phage at 8 h Fermentation periods (h) 72 96 96 80 80 80 72 2KGA concentration (g/L) 178.45 ± 1.41 144.98 ± 1.61 150.79 ± 1.42 168.85 ± 1.95 159.89 ± 2.52 163.59 ± 1.55 171.34 ± 1.25 percent conversion(%) 91.99 ± 0.71 74.73 ± 0.83 77.73 ± 0.74 87.04 ± 1.00 82.42 ± 1.30 84.32 ± 0.80 88.32 ± 0.64 Total productivity (g/L.h) 2.48 ± 0.02 1.51 ± 0.01 1.57 ± 0.01 2.11 ± 0.03 2.00 ± 0.30 2.04 ± 0.02 2.38 ± 0.01 Maximum productivity (g/L.h) 2.61 ± 0.13 1.71 ± 0.17 1.79 ± 0.04 2.26 ± 0.05 2.15 ± 0.17 2.21 ± 0.06 2.54 ± 0.04 Yield (g/g) 0.99 ± 0.01 0.81 ± 0.01 0.84 ± 0.01 0.94 ± 0.01 0.89 ± 0.01 0.91 ± 0.01 0.95 ± 0.01 Conclusions The isolation and characterization of a specifically-infecting phage KSL-1 to 2KGA producer Ps.