There are instances where regulation differs between

closely related bacteria [6–8] so how conserved is regulation, especially global regulation, within a species? We approach this question by measuring the concentration of two cellular components with global regulatory roles in multiple members of the same species. We focus on two factors with complementary functions in switching between vegetative growth and stress-related gene expression. The RpoS sigma factor (σS), responds to stress and shifts transcription away from vegetative growth and towards stress resistance [9–12]. Higher levels of RpoS in stressed or stationary-phase cells alter STA-9090 concentration expression of several hundred genes [13, 14]. The alarmone ppGpp [15] also accumulates in bacteria undergoing stress, such as amino acid, carbon or phosphate

limitation [16–19]. Accumulation of ppGpp triggers the stringent response and a radical decrease in ribosome and protein synthesis, even leading to growth arrest [20, 21]. ppGpp and σS co-operate both mechanistically and strategically under stress and expression of σS-controlled genes is partly dependent on ppGpp [22, 23]. The level of ppGpp also controls the amount of σS in the cell, as ppGpp increases by several-fold the cellular concentration of σS during nutritional stress or in the stationary phase. The absence of ppGpp impairs Belinostat or severely delays the accumulation of σS [9] and ppGpp positively affects the efficiency of rpoS translation under stress conditions as well as rpoS basal expression under conditions of optimal growth [24, 25]. The response to phosphate starvation additionally involves stabilisation of RpoS protein sensed through SpoT [19]. At several levels then, ppGpp is intertwined with rpoS regulation and here we investigate the conservation of the level Ribose-5-phosphate isomerase of these regulators across the species E. coli. This study was prompted by several indications that RpoS and ppGpp were subject

to strain variation. The rpoS gene is polymorphic in isolates of E. coli [26]. Recently, variations in ppGpp levels were also observed between laboratory strains of E. coli due to spoT mutations [21]. However, the assumption that rpoS is subject to extensive variation has been challenged [27]. These authors claimed that the endogenous RpoS levels are actually fairly conserved in E. coli. They also noted that the trade-off hypothesis was originally based on only two high-RpoS strains in [28]. Here, we study the hypothesis that stress-related gene expression is variable across the species E. coli Poziotinib cost because it involves a trade-off in the expression of genes related to stress resistance and vegetative growth [11].