Vacuolar type is common. B.hominis growth curve present ‘S’ shape, went through three growth stages: incubation period, the logarithmic growth phase and stagnation. 3. Results of electron microscopic enzyme cytochemistry showed that the acid phosphatase enzyme, adenosine triphosphate

(ATP enzyme), thiamine pyrophosphate enzyme (TPP enzyme), peroxidase were be positive, glucose-6-phosphatase, cytochrome oxidase were negative. Conclusion: The, B.hominis contain part of the organelle marker enzyme. Key Word(s): 1. Blastocystis hominis; 2. organelle enzyme; 3. DMEM medium; 4. morphology; Presenting Author: HAO NING-BO Additional Authors: YANG SHI-MING Corresponding Navitoclax Author: YANG SHI-MING Affiliations: Department of Gastroenterology, XinQiao

Hospital Objective: The role of the MIF −173 G/C gene polymorphism in the incidence of inflammatory bowel disease (IBD) is controversial. Methods: We performed a meta-analysis including 2084 cases and 2284 controls for whom Alpelisib the MIF −173 G/C polymorphism was genotyped. Results: Results show MIF −173 G/C gene polymorphism are associated with IBD in both the recessive model (CC vs. GC+GG) and the codominant model (CC vs. GG). In the stratified analysis by ethnicity, a significantly increased risk was observed in Asians in the recessive model and codominant model. In the subgroups of ulcerative colitis (UC) and Crohn’s disease (CD), significant differences were observed in UC in the recessive model and the codominant model. In the stratified analysis of ethnicity for UC, significant differences were observed in Asians for the recessive model Conclusion: The current meta-analysis suggested that the MIF −173 G/C polymorphism contributed to the susceptibility of IBD, especially for UC in Immune system Asians. Key Word(s): 1. Macrophage migration; 2. G/C polymorphism; 3. IBD; 4. meta-analysis; Presenting Author: WEI HOU Additional Authors: WEI LU Corresponding Author: WEI HOU Affiliations: Tianjin Second People’s Hospital and Tianjin Institute of Hepatology Objective: Our previous work showed that GW182, a processing body (P-body) component,

is essential for hepatitis C virus (HCV) replication (HEPATOLOGY 2013;57:70–80). The aim of this study was to further investigate the expression and subcellular localization of recombinant GW182-RFP in human hepatocellular carcinoma cell line Huh7 cells. Methods: The TNRC6A (GW182) gene encompassing nucleotides 115–6000 (5886bp) (GenBank Accession No. NM_014494) was amplified using primers with engineered restriction sites to facilitate the fusion of TNRC6A ‘inframe’ to the N terminus of red fluorescent protein (RFP) in the pTagRFP-N vector (Everogen) to construct fusion protein expression plasmid termed pTNRC6A-RFP. Forward primer (5′-GCGAGCTCATGAGAGAATTGGAAGCTAAAG-3′) containing Sac I restriction site and reverse primer (5′-CGCCGCGGCATGGACTCTCCACCCCC-3′) containing Sac II restriction site were synthesized.