We observed no selleck chemicals llc evidence, but can not exclude, the possibility that clinical isolates may have acquired specific pathogenicity factors beyond T3SS on plasmids or other mobile elements, as has been reported for phytopathogenic

strains [44,45]. The T3SS discovered in some strains, however, was found to be more closely related to that in biocontrolPseudomonasspp. indicating a non-pathogenic function [57]. Furthermore, only one clinical isolate had a T3SS gene compared to six environmental isolates. Comparison between the completed genome of biocontrol strain C9-1 and the in progress genome sequencing of the clinical type strain ofP. agglomeransLMG 1286T(T.H.M. Smits, B. Duffy et al., unpublished data) indicates that several features including CHIR-99021 mouse antibiotic production (revealed OSI-027 cell line by the presence ofpaaABCgenes [58]), and nectar sugar utilization as a sole carbon source are generally associated with antagonistic activity. Our results demonstrate, however, that while many biocontrol strains have such traits, not all do and thus these are not universal features of biocontrol potential. Also, we have demonstrated

for the first time the presence of the antibiotic biosynthetic genespaaABCin clinical strains, indicating that these may not be unique signatures of biocontrol isolates. What if any role pantocin may contribute to animal associations remains to be determined. There was no difference in growth at 37°C Celastrol between clinical and biocontrol isolates, with both types of strains growing poorly at this temperature compared to growth at 27°C, and reinforcing the weakness of this criteria to determine pathogenicity. Returning to the fundamental problem of insufficient confidence in identification procedures, we have shown that specific gene sequences (such asgyrBrather than 16S rDNA) are more robust than biochemical identification regardingP. agglomerans. The several reports ofP. agglomeransfrom clinical

literature upon which biosafety decisions have been based all lack a clear establishment of this species as a primary and singular cause of disease. With rare exception such isolates are not available for precise taxonomic confirmation and detailed clinical histories are typically absent for individual strains. We conducted a small survey of three clinical diagnostic laboratories in Switzerland and found thatP. agglomeransis infrequently recovered.P. agglomeranswas identified, predominantly as a polymicrobial co-isolate in patients, 21 times in the last four years at the ICM in Bellinzona (M. Tonolla, personal communication) and six times in the last three years at the Kantonsspital Lucerne (M. Hombach, personal communication).